Dear Geneious Team,
A very helpful feature would be to be able to highlight Restriction Endonucleases with impaired cutting by procaryotic(actualy E. coli KI) DNA methylation.
This is quite a hassle in the lab for cloning, especially for our newer lab members.
The info about methylation sensitivity of RE's is on REbase but not easily available.
For an example:
Find an extensive article here:
If you could just implement the E. coli methylation in some way that would already be helpful.
A possible workaround would be to implement Dam, Dcm & EcoKI as "Enzymes" to search the DNA for. If overlapping occurs one would still have to look if cutting is impaired or not. Not much, but still better than nothing.
I would also be happy to use a Plugin if that's easier.
I mean you don't have to cover all organisms since almost everybody uses the same E. coli strains for cloning.