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assembly by name for next generation sequencing

I am using Geneious server to assemble the next generation sequencing data that are pair end reads. My question is which part of name should be chosen in assembly by name in Geneious? I will be grateful if somebody answers to my question. Thank you! Best regards, F.S

Fariba Shafiei

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Assembly by name is not normally suitable for NGS data.  If you have a single paired read dataset, then you need to pair the reads by using "Set paired reads" under the Sequence menu.  You can then run the assembly on the paired file.  If you have multiple datasets that you want to assemble separately, run Set paired reads for each pair of reads and then select all the paired read files and use "Assemble each sequence list separately".  

 

Hilary Miller 0 votes